@article{Kazuhito Murakami_Akiko Inoue-Kohama_2016, title={HORIZONTAL TRANSFER OF PLASMID DNA BETWEEN DIFFERENT BACTERIA SPECIES UNDER MICROBIAL INTERACTIONS }, volume={12}, url={https://geomatejournal.com/geomate/article/view/1236}, abstractNote={<p>Horizontal transfer of plasmid DNA was investigated under phytoplankton metabolites / <br>zooplankton predation exposure condition, to obtain some basic information about the prosperity and decay <br>of GMO (genetically modified microorganisms) in filed release was investigated in this study. Escherichia <br>coli HB101, E.coli C600, E.coli S17-1, Pseudomonas aeruginosa PAO1 (gram-negative) and Bacillus cereus<br>MC (gram-positive) as recipient strain of plasmid DNA, E.coli HB101/pBR325, E.coli C600/RP4 and E.coli<br>S17-1/pSUP104 as donor of plasmid DNA, were supplied. As phytoplankton, Microcystis aeruginosa<br>(cyanophyceae), Melosira varians (bacillariophyceae) and Scenedesmus quadricauda (chlorophyceae) <br>collected from Lake Tega as donor of metabolites, were supplied. As zooplankton, Tetrahymena pyriformis<br>(cilliata) and Philodina erythrophthalma (rotifer) collected from Lake Tega as predator were supplied. The <br>results can be concluded as follows; 1) Phytoplankton metabolites leads acceleration of horizontal transfer <br>between not only same strains but also different strain in spite of whether transmissible or not, 2) <br>Zooplankton predation leads decrease of bacterial individual number and horizontal transfer of plasmid DNA, <br>and 3) Horizontal plasmid DNA transfer is influenced greatly, because the natural ecosystem includes <br>phytoplankton as producer and zooplankton as consumer in the same time.</p>}, number={30}, journal={GEOMATE Journal}, author={Kazuhito Murakami and Akiko Inoue-Kohama}, year={2016}, month={Nov.}, pages={84–89} }