TOXICITY OF SIO2, TIO2 AND CEO2 NANOPARTICLES EVALUATED USING THE BIOLUMINESCENCE ASSAY
Keywords:Bacterial bioluminescence, Nanoparticles, Silicon dioxide, Dioxide of titanium, Cerium dioxide
The development of technology and industry has made available a variety of different nanomaterials.
Particularly popular in practical use have been silicon dioxide nanoparticles and titanium and cerium dioxides.
However, it is necessary to evaluate the biological activity of nanoparticles from the point of view of toxicology.
Despite the active use of nanoparticles of silicon dioxide and dioxides of titanium and cerium, little is known about
their toxic effects on living organisms. This work presents a study of three various nanoparticles of silicon dioxide
and dioxides of titanium and cerium. For investigations of nanoparticles morphology and their contact with E. coli
K12 TG1 cells, suspensions alone or previously mixed with nanoparticles, were applied to freshly prepared mica.
To assess the activity of the nanoparticles against E. coli K12 TG1 cells, we used a previously described version
of bioluminescent analysis. TiO2 particles having smaller dimensions were characterized by a higher toxicity
compared to SiO2. These results characterized TiO2 (I) as toxic and confirm the statement that size matters. Metal
particles of CeO2 in the studied concentrations of 0.1–0.000195 M did not cause changes in the dynamics of
bacterial bioluminescence. The results were confirmed by studies carried out using lux-biosensors: E. coli K12
MG1655 pkatG'::lux (for the detection of peroxide of hydrogen) and E. coli K12 MG1655 psoxS'::lux ( for the
detection of superoxide anion)) for the assessment of oxidative damage.