MORPHOFUNCTIONAL CHARACTERISTICS AND ELEMENTAL COMPOSITION OF RAT LIVER UNDER DIFFERENT NUTRIENT PROVISION

Abstract

The article presents research of structural and functional changes in the liver in the conditions
of modeling of micronutrient deficiencies in the organism of experimental animals with subsequent inclusion
of complex minerals into their food ration. The object of the study was the liver of rats «Wistar». The
elemental composition of biological substrates has been studied using atomic emission and mass
spectrometry (ICP-AES and ICP-MS) in the test laboratory ANO «Center for Biotic Medicine». It has been
established that the addition of selenium to the ration increases the concentration of this element in the liver
by 15,0% (p≤0,05) against the background of lowering nickel by 50,0% (p≤0,05) in the experimental group I
relative to control. When you turn on zinc into the ration, the concentration of it increases by 14,3% (p≤0,05)
in the experimental group II relative to the control. Inclusion of iodine in the ration contributed to the
increase of the iodine concentration by 4,52% in the experimental group III relative to the control group.
Thus, the intake by the animal organism of toxic elements (lead and cadmium salts) leads to a change in the
structural elements of the liver, resulting in depletion of hepatocyte in glycogen, or its complete
disappearance, in offensive vacuolization and necrosis of hepatocytes and in changes in vessels diameter
microvasculature, i.e. structural and functional changes in the liver are identified. Introduction into the ration
of complex of essential trace elements (I, Se, Zn) involves an increase in functional activity of the liver.