DETERMINATION OF PHYTOCHEMICAL COMPOUND FROM Spirogyra sp. USING ULTRASONIC ASSISTED EXTRACTION

Abstract

Currently, the use of phytochemical compounds from macro algae for cosmetic and pharmaceutical
purposes is popular. This study aimed to evaluate ultrasound assisted-extraction (UAE) method on phytochemical
components (gallotannin, total flavonoids content, total saponin content and total phenolic compounds) in Spirogyra
sp.. The ultrasonic devices with same input power of 5 watts and varying 3 frequencies (45 kHz, 210 kHz and 1
MHz) of UAE coupled with 3 solvents (ethanol, methanol and acetone) were investigated. Treated samples were
collected after sonication for 120 min. The results revealed that UAE method showed the highest performance in
yield extraction compared with hot water. The highest yields of gallotannin and total flavonoids content were
obtained by sonication at 45 kHz with 2.7 mg/g and 3.5 mg/g acetone but the highest yield of total phenolic
compounds was found when sonicated at 45 kHz with methanol and total saponin content was found with ethanol.
Thus, UAE at 5 watts, 45 kHz. showed the best result in yields extraction of those phytochemical compounds. While
the suitable solvents for phytochemical compounds extraction differed and depended on the purpose and
phytochemical type. UAE in combination with acetone had a suitable effect on P. digitatum inhibition while UAE in
combination with acetone, ethanol and methanol showed non significantly different on E.coli inhibition.