ORGANIC SOLVENT-RESISTANT PROPERTIES OF PROTEINS ADSORBED ONTO BIOMASS CHARCOAL POWDER

Authors

  • Hidetaka Noritomi
  • Jumpei Nishigami
  • Nobuyuki Endo
  • Satoru Kato
  • Katsumi Uchiyama

Keywords:

Biomass Charcoal Powder, Protein, Adsorption, Organic Solvent Resistance, α-Chymotrypsin

Abstract

We have found out that biomass charcoal powder (BCP), which is prepared from plant biomass
wastes by pyrolysis at low temperatures under nitrogen atmosphere, imparts organic solvent resistance to
proteins by using BCP as a protein carrier. α-Chymotrypsin (α-CT), which was used as a model protein, was
sufficiently adsorbed onto the surface of BCP. When free α-CT and BCP-adsorbed α-CT were immersed in
acetonitrile containing 5%(v/v) water, they catalyzed the transesterification of N-acetyl-L-tyrosine ethyl ester
(N-Ac-Tyr-OEt) with n-butanol (BuOH) to produce N-acetyl-L-tyrosine butyl ester (N-Ac-Tyr-OBu), which
did not proceed in an aqueous solution, where α-CT worked as a hydrolase. The initial rate of transesterification
catalyzed by BCP-adsorbed α-CT was strongly dependent upon the kind of BCP, and was about fifty times
higher than that catalyzed by free α-CT when bamboo charcoal powder was used as a carrier.

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Published

2016-12-14

How to Cite

Hidetaka Noritomi, Jumpei Nishigami, Nobuyuki Endo, Satoru Kato, & Katsumi Uchiyama. (2016). ORGANIC SOLVENT-RESISTANT PROPERTIES OF PROTEINS ADSORBED ONTO BIOMASS CHARCOAL POWDER. GEOMATE Journal, 12(30), 140–145. Retrieved from https://geomatejournal.com/geomate/article/view/1249

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