EFFECT OF ADSORPTION CONDITION ON THERMAL STABILITY OF PROTEINS ADSORBED ONTO BIOMASS CHARCOAL POWDER

Abstract

Authors have found out that bamboo charcoal powder (BCP), which is prepared from bamboo
wastes by pyrolysis at low temperatures, is very useful as a carrier for the thermal stabilization of proteins. Hen
egg white lysozyme (HEWL) and α-chymotrypsin (CT) were effectively adsorbed onto BCP. The thermal
stability of BCP-adsorbed HEWL was strongly dependent upon the adsorption conditions such as solution pH,
ionic strength, and temperature. Moreover, writers have revealed the heat-resistant mechanism on the basis of
the structure of HEWL. Likewise, the thermal stability of BCP-adsorbed CT could be enhanced by selecting the
adsorption condition.